Fresh leafy green vegetables associated with multidrug resistant

Objective: To detect the occurrence of diarrheagenic E. coli, contamination andantibiotic resistance of E. coli isolates from fresh leafy vegetables presented inJordanian retail markets.Materials and Methods: A total 150 fresh leafy green vegetable samples werecollected from different markets in Amman and Al-Zarqa in Jordan over the fivemonths; June through October, 2010. All samples were prepared in sterile normalsaline and cultured on MacConkey agar at 37°C for 24 hours. E. coli isolates werelater identified by biochemical tests and the isolates were investigated for presenceof diarrheagenic E. coli types and antimicrobial resistance genes, integronsand plasmids.Results: A total of 61/150 (40.6%) of fresh leafy green vegetable samples werefound contaminated with E. coli. The highest contamination with E. coli occurredin parsley and lowest in Lettuce. A range of 3-41% of E. coli isolates were resistantto 7 common antibiotics used often in Jordan. A total of 17 (27.8%) E. coli isolateswere resistant to three or more antimicrobial agents and considered to be multidrugresistant. All these 17 (100%) carry at least one plasmid with a common sizes(7.3 and 54.3 Kb), 14 (82%) isolates were positive for class 1 integrons, 12 (70.6%),and 2 (11.8%) isolates were positive for Tet A and Tet B genes, respectively. A totalof 15 (88.2%) isolates were positive for sul 2 and 4 (23.5%) isolates for sul1genes.Conclusion: This study demonstrates the absence of common diarrheagenic E. coli,but indicates widespread of antimicrobial resistance in E. coli contaminating freshgreen produce which may increase the reservoir of antimicrobial resistance in theintestinal tract of Jordanian population

via The International Arabic Journal of Antimicrobial Agents

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